Chercheurs

Sciences de la Vie / KOURILSKY, PHILIPPE

caput

Position

Professeur au Collège de France (chaire d'Immunologie moléculaire) et Professeur associé à l'Institut Pasteur. Il a rejoint l'Institut Pasteur où il dirige l'Unité de Biologie Moléculaire du Gène.

Membre de l'Académie des Sciences et de l'Academia Europaea.

Thèmes de recherche

étude de la structure, de l'expression et de la régulation des gènes des organismes supérieurs, notamment celui de l'ovalbumine de la poule ; étude des gènes codant pour les antigènes majeurs d'histocompatibilité, de leur polymorphisme, du mécanismes qui règlent leurs expressions, puis à expliciter leur mode d'action dans le déclenchement des réponses immunes, particulièrement dans l'activation des cellules tueuses spécifiques. Contribution :à la définition de plusieurs protocoles de vaccination anti-tumorales et à l'étude de la composition des répertoires immunitaires et à l'analyse de leur variabilité individuelle.

Prix et récompenses

1986 : Prix international d'Immunopathologie
1990 : Prix Lacassagne du Collège de France
2000 : Prix International de la recherche en sciences médicales

Publications

Principaux ouvrages et articles
Outre quelque 300 publications dans des revues internationales,
- Les Artisans de l'Hérédité, éditions Odile Jacob, Paris (1987)
- La Science en Partage, éditions Odile Jacob, Paris (1998)
- Le Principe de Précaution, La Documentation Française, Ed. Odile Jacob (2000)

Articles majeurs
- KOURILSKY P., LUZZATI D.: Etude par sédimentation de zone des produits de transcription des fonctions "précoces" de lambda. J. Mol. Biol. (1967) 25, 357-362.

- KOURILSKY P., MARCAUD L., SHELDRICK P., LUZZATI D., GROS, F.: Studies on the messenger RNA of bacteriophage lambda. I) Various species synthetized early after induction of prophage. Proc. Natl. Acad. Sci., USA (1968) 61, 1013-1020.

- KOURILSKY P. : Lysogenization by bacteriophage lambda and the regulation of lambda repressor synthesis. Virology (1971) 45, 853-857 .

- MARCAUD L., PORTIER M.-M., KOURILSKY P., BARRELL B.G., GROS F.: A low molecular weigth species of RNA synthetized early after induction of the prophage lambda. J. Mol. Biol. (1971) 57, 247-261.

- ROUGEON F., KOURILSKY P., MACH, B.: Insertion of a rabbit beta-globin gene sequence into an E. coli plasmid. Nucl. Acids Res. (1975) 2, 2365-2378.

- SANZEY B., MERCEREAU-PUIJALON O., TERNYNCK T., KOURILSKY P.: Methods for identification of recombinants of phage lambda. Proc. Natl. Acad. Sci., USA (1976) 73, 3394-3397.

- MERCEREAU-PUIJALON O., KOURILSKY P.: Escape synthesis of beta-galactosidase under the control of bacteriophage lambda. J. Mol. Biol. (1976) 108, 733-751.

- KOURILSKY P., GROS D., ROUGEON F., MACH B.: Transcription of a mammalian sequence under phage lambda control. Nature (1977) 267, 637-639.

- GARAPIN A.-C., LE PENNEC J.-P., ROSKAM, W., PERRIN F., CAMI B., KRUST A., BREATHNACH R., CHAMBON P., KOURILSKY P.: Isolation by molecular cloning of a fragment of the split ovalbumin gene. Nature (1978) 273, 349-354.

- GARAPIN A.-C., CAMI B., ROSKAM, W., KOURILSKY P., LE PENNEC J.-P., PERRIN F., GERLINGER P., COCHET M., CHAMBON P.: Electron microscopy and restriction enzyme mapping reveal additional intervening sequences in the chicken ovalbumin split gene. Cell (1978) 14, 629-639.

- MERCEREAU-PUIJALON O., ROYAL A., CAMI B., GARAPIN A.-C., KRUST A., GANNON F. & KOURILSKY P.: Synthesis of an ovalbumin-like protein by E. coli K12 harbouring a recombinant plasmid. Nature (1978) 275, 505-510.

- COLBERE-GARAPIN F., CHOUSTERMAN S., HORODNICEANU F., KOURILSKY P., GARAPIN A.-C.:
Cloning of the active thymidine kinase gene of herpes simplex virus type I in E. coli K12. Proc. Natl. Acad. Sci. USA (1979) 76, 3755-3759.

- MERCEREAU-PUIJALON O., KOURILSKY P.: Introns in the chicken ovalbumin gene prevent ovalbumin synthesis in E. coli K12. Nature (1979) 279, 647-649.

- ROYAL A., GARAPIN A.-C., CAMI B., PERRIN F., MANDEL J.-L., LE MEUR M., BREGEGERE F., GANNON F., LE PENNEC J.-P., CHAMBON P. & KOURILSKY P.: The ovalbumin gene region: common features in the organization of three genes expressed in chicken oviduct under hormonal
control. Nature (1979) 279, 125-132.

- MERCEREAU-PUIJALON O., LACROUTE F., KOURILSKY P.: Synthesis of a chicken ovalbumin-like protein in the yeast Saccharomyces cerevisiae. Gene (1980) 11, 163-167.

- BREGEGERE F., ABASTADO J.-P., KVIST S., RASK L., LALANNE J.-L., GAROFF H., CAMI B., WIMAN K., LARHAMMAR D., PETERSON P.A., GACHELIN G., KOURILSKY P., DOBBERSTEIN B. : Structure of the C-terminal half of two H-2 antigens deduced from their cloned mRNA sequences. Nature (1981) 292, 78-81.

- CAMI B., BREGEGERE F., ABASTADO J.-P. & KOURILSKY P.: Multiple sequences related to classical histocompatibility antigens in the mouse genome. Nature (1981) 291, 673-675.

COLBERE-GARAPIN F., HORODNICEANU F., KOURILSKY P., GARAPIN A.-C.: A new dominant hybrid selective marker for higher eukaryotic cells. J. Mol. Biol. (1981) 150, 1-14.

- GARAPIN A.-C., COLBERE-GARAPIN F., COHEN-SOLAL M., HORODNICEANU F., KOURILSKY P.: Expression of the Herpes simplex virus type I thymidine kinase gene in E. coli. Proc. Natl. Acad. Sci. USA (1981) 78, 815-819.

- JORDAN B.R., BREGEGERE F., KOURILSKY P.: Human HLA gene segment isolated by hybridization with mouse H-2 cDNA probes. Nature (1981) 290, 521-523.

- KVIST S., BREGEGERE F., RASK L., CAMI B., GAROFF H., DANIEL F., WIMAN K., LARHAMMAR D., ABASTADO J.-P., GACHELIN G., PETERSON P.A., DOBBERSTEIN B., KOURILSKY P.: A cDNA clone coding for part of mouse H-2d major histocompatibility antigens. Proc. Natl. Acad. Sci. USA (1981) 78, 2772-2776.

- VAN DER WERF S., BREGEGERE F., KOPECKA H., KITAMURA N., ROTHBERG P.G., KOURILSKY P., WIMMER E., GIRARD M.: Molecular cloning of the genome of poliovirus type I. Proc. Natl. Acad. Sci. USA (1981) 78, 5983-5987.

- MORELLO D., DANIEL F., BALDACCI P., CAYRE Y., GACHELIN G., KOURILSKY P.: Absence of significant H-2 and beta.2 microglobulin mRNAs expression by mouse embryonal carcinoma cells. Nature (1982) 296, 260-262.

- BALDACCI P., POZO F., GISSELBRECHT S., KOURILSKY P.: Altered transcription of genes coding for class I histocompatibility antigens in murine tumor cells. J. Exp. Med. (1983) 158, 1294-1306.

- DANIEL F., MORELLO D., LE BAIL O., CHAMBON P., CAYRE Y., KOURILSKY P.: Structure and
expression of the mouse beta.2-microglobulin gene isolated from mouse somatic and non-expressing teratocarcinoma cells. EMBO J. (1983) 2, 1061-1065.

- LALANNE J.-L., TRANSY C., GUERIN S., DARCHE S., MEULIEN P. & KOURILSKY P.: Expression of class I genes in the major histocompatibility complex: identification of eight disting mRNAs in DBA/2 mouse liver. Cell (1985) 41, 469-478.

- ISRAEL A., KIMURA A., FOURNIER A., FELLOUS M. & KOURILSKY P.: Interferon response sequence potentiates activity of an enhancer in the promoter region of a mouse H-2 gene. Nature (1986) 322, 743-746.

- KIMURA A., ISRAEL A., LE BAIL O. & KOURILSKY P.: Detailed analysis of the mouse H-2Kb promoter : enhancer-like sequences and their role in the regulation of class I gene expression. Cell (1986) 44, 261-272.

- KOURILSKY P., CHAOUAT G., RABOURDIN-COMBE C. & CLAVERIE J.-M.: Working principles in the immune system implied by the "peptidic self" model. Proc. Natl. Acad. Sci., USA (1987) 84, 3400-3404.

- PLATA F., LANGLADE-DEMOYEN P., ABASTADO J.-P., BERBAR T. & KOURILSKY P.: Retrovirus antigens recognized by cytolytic T lymphocy-tes activate tumor rejection in vivo. Cell (1987) 48, 231-240.

- KIERAN M., BLANK V., LOGEAT F., VANDEKERCKHOVE J., LOTTSPEICH F., LE BAIL O., URBAN M.B., KOURILSKY P., BAEUERLE P.A. & ISRAEL A.: The DNA-binding subunit of NF-kappa.B is identical to factor KBF1 and homologous to the rel oncogene product. Cell (1990) 62, 1007-1018.

- CIBOTTI R., KANELLOPOULOS J.M., CABANIOLS J.-P., HALLE-PANNENKO O., KOSMATOPOULOS K., SERCARZ E. & KOURILSKY P.: Tolerance to a self-protein involves its immunodominant but not subdominant determinants. Proc. Natl. Acad. Sci., USA, (1992) 89, 416-420.

- PANNETIER C., COCHET M., DARCHE S., CASROUGE A., ZÖLLER M. & KOURILSKY P.: The sizes of the CDR3 hypervariable regions of the murine T-cell receptor beta chains vary as a function of the recombined germ-line segments. Proc. Natl. Acad. Sci., USA (1993) 90, 4319-4323.

- CIBOTTI R., CABANIOLS J.-P., PANNETIER C., DELARBRE C., VERGNON I., KANELLOPOULOS J.M. & KOURILSKY P. : Public and private Vbeta TcR repertoires against hen egg white lysozyme (HEL) in non-transgenic versus HEL transgenic mice. J. Exp. Med. (1994) 180, 861-872.

- ABASTADO J.-P., LONE Y.-C., CASROUGE A., BOULOT G. & KOURILSKY P.: Dimerization of soluble MHC-peptide complexes is sufficient for activation of T-cell hybridoma and induction of unresponsiveness. J. Exp. Med. (1995) 182, 439-447.

- LEVRAUD J.-P., PANNETIER C., LANGLADE-DEMOYEN P., BRICHARD V. & KOURILSKY P.: Recurrent T cell receptor rearrangements in the cytotoxic T lymphocyte response in vivo against the P815 murine tumor. J. Exp. Med. (1996) 183, 439-449.

- BOUSSO P., CASROUGE A., ALTMAN J.D., HAURY M., KANELLOPOULOS J., ABASTADO J.-P. & KOURILSKY P.: Individual variations in the murine T cell response to a specific peptide reflect variability in naive repertoires. Immunity (1998) 9, 169-178.

- DELON J., GREGOIRE C., MALISSEN B., DARCHE S., LEMAITRE F., KOURILSKY P., ABASTADO J.-P.,
TRAUTMANN A.: CD8 expression allows T cell signalling by monomeric peptide-MHC complexes.
Immunity (1998) 9, 467-473.

- GAPIN L., FUKUI Y., KANELLOPOULOS J., SANO T., CASROUGE A., MALIER V., BEAUDOING E., GAUTHERET D., CLAVERIE J.-M., SASAZUKI T. & KOURILSKY P.: Quantitative analysis of the T cell repertoire selected by a single peptide/Major Histocompatibility Complex. J. Exp. Med. (1998) 187, 1871-1883.

- APOSTOLOU I., TAKAHAMA Y., BELMANT C., KAWANO T., HUERRE M., MARCHAL G., CUI J., TANIGUCHI M., NAKAUCHI H., FOURNIE J.-J., KOURILSKY P., GACHELIN G.: Murine natural KT cells contribute to the granulomatous reaction caused by mycobacterial cell walls. Proc. Natl. Acad. Sci., USA (1999) 96, 5141-5146.

- ARSTILA T.P., CASROUGE A., BARON V., EVEN J., KANELLOPOULOS J., KOURILSKY P.: A direct estimate of ther human alpha,beta T cell receptor diversity. Science (1999) 286, 958-961.

- BOUSSO P., LEVRAUD J.-P., KOURILSKY P., ABASTADO J.-P.: The composition of a primary
T cell response is largely determined by the timing of recruitment of individual T cell clones. J. Exp. Med. (1999) 189, 1591-1600.

- ARSTILA T., ARSTILA T.P., CALBO S., SELZ F., MALASSIS-SERIS M., VASSALLI P., KOURILSKY P., GUY-GRAND, D.: Identical T cell clones are located within the mouse gut epithelium and Lamina propria and circulate in the thoracic duct lymph. J. Exp. Med. (2000) 191, 823-834.

- BOUNEAUD C., KOURILSKY P., BOUSSO, P.: Impact of negative selection on the T cell repetoire reactive to a self-peptide: a large fraction of T cell clones escapes negative deletion. Immunity (2000) 13, 829-840.

- BOUSSO P, WAHN V., DOUAGI I, HORNEFF G., PANNETIER C., LE DEIST, ZEPP F., NIEHUES T.,
KOURILSKY P., FISCHER A., de SAINT-BASILE G. : Diversity, functionality and stability of the T cell repertoire derived in vivo from a signle human T cell precursor. Proc. Natl. Acad. Sci., USA (2000) 97, 274-278.

- FUKUI Y., OONO, T., CABANIOLS J.-P., NAKAO K., HIROKAWA K;, INAYOSHI A., SANUI T., KANELLOPOULOS J., IWATA E., NODA M., KATSUKI M., KOURILSKY P., SASAZUKI T.: Diversity of T cell repertoire shaped by a single peptide ligand is critically affected by amino acid residue at a T cell receptor-contact. Proc. Natl. Acad. Sci., USA (2000) 97, 13760-13765.